RESULTS OBTAINED IN THE IMPLEMENTATION OF THE PROJECT
КП-06-Н26-6


  • The populations of Helichrysum arenarium and Alkanna tinctoria have been revised and inventoried and maps of their distribution in Bulgaria have been made.


    		•
    As a result of the conducted researches it was established that the populations of Alkanna tinctoria tinctoria in Bulgaria are localized in four floristic regions of the country: Strumska valley, Southern Pirin and Eastern Rhodopes and Danube plain, as the majority are located in Strumska valley, which is the main region of distribution of the species / the others are represented by one population each /.
    Distribution of Alkanna tinctoria in Bulgaria : •/red circle / confirmed localities, ● / black circle / unconfirmed ocalities

    As a result of the conducted researches it was established that in Bulgaria Helichrysum arenarium is represented by six populations, located in a limited area in the northeastern part of the country, in a narrow strip between the towns of Shumen and Varna/.
    Distribution map of Helichrysum arenariumin Bulgaria: •/red circle / confirmed localities, ● / black circle / unconfirmed ocalities



  • Ex situ collections of Alkanna tinctoria, Primula veris and Helichrysum arenariumwere created on the experimental areas of IBEI, BAS and AU, Plovdiv
    and the greenhouse of IBEI, BAS and of and of Arctostaphilus uva ursi in the greenhouse of IBEI, BAS.

    • Alkanna tinctoria
    Primula veris
    Helichrysum arenarium
    Arctostaphylos uva-ursi


  • The size of the genome in the target species has been determined



    • Helichrysum arenarium


    Alkanna tinctoria


    Primula veris


    Arctostaphilus uva ursi


  • Successful attempts for hydroponic cultivation have been made:


    • Hydroponic cultivation of Alkanna tinctoria



    Hydroponically grown plants from Alkanna tinctoria on the Cutting board hydroponic system


  • In vitro cultures from Helichrysum arenarium were initiated






  • The main features of the male and female generative sphere are revealed and
    the reproductive potential of Alkanna tinctoria: is assessed:



    • Evaluation of pollen viability and peculiarities of anther structure and development of the male gametophyte


    Features of the structure of the ovary and the development of the female gametophyte


    Evaluation of seed viability



  • The reproductive potential of Helichrysum arenarium. was assessed by
    testing the viability of pollen and seeds


    • Pollen viability


    Seed viability



  • The peculiarities of the male and female generative sphere have been established and
    the reproductive potential of Primula veris has been assessed.



    • Male generative sphere



    Structure of the ovary and the pistil



    Development of the female gametophyte



    Development of the female gametophyte



    Seed viability: A.-seed with viable embryo and endosperm; B.- seed with nonviable embryo and endosperm; C.- empty seed.



  • A comparative analysis of the metabolic profile of aboveground and underground parts of Alkanna tinctoria
    by GC / MS, with emphasis on the content of alkanin was made



    • A comparative analysis of the metabolic profile



  • Metabolites in acetone exudates from Helichrysum arenarium flower baskets have been identified



    • Identified metabolites in acetone exudates


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